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KMID : 1161420160190070663
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Journal of Medicinal Food
2016 Volume.19 No. 7 p.663 ~ p.669
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Inhibition of TNF-α, IL-1α, and IL-1β by Pretreatment of Human Monocyte-Derived Macrophages with Menaquinone-7 and Cell Activation with TLR Agonists In Vitro
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Pan Min-Hsiung
Maresz Katarzyna
Lee Pei-Sheng
Wu Jia-Ching
Ho Chi-Tang
Popko Janusz
Mehta Dilip S.
Stohs Sidney J.
Badmaev Vladimir
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Abstract
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Circulatory markers of low-grade inflammation such as tumor necrosis factor-alpha (TNF-α), interleukin-1 alpha (IL-1α), and interleukin-1 beta (IL-1β) positively correlate with endothelial damage, atheroma formation, cardiovascular disease, and aging. The natural vitamin K2-menaquinone-7 (MK-7) added to the cell culture of human monocyte-derived macrophages (hMDMs) at the same time as toll-like receptor (TLR) agonists did not influence the production of TNF-α. When the cells were pretreated up to 6?h with MK-7 before treatment with TLR agonists, MK-7 did not inhibit significantly the production of TNF-α after the TLR activation. However, 30?h pretreatment of hMDMs with at least 10?μM of MK-7 effectively and dose dependently inhibited the proinflammatory function of hMDMs. Pretreatment of hMDMs with 10?μM of MK-7 for 30?h resulted in 20% inhibition of TNF-α production after lipopolysaccharide (LPS) activation (P?
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KEYWORD
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cardiovascular, inflammation, menaquinone-7, MenaQ7® Crystals, TNF-α
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